On February 6, 2016, a team of five Marine Biology Research scholars set out to sequence the genetic barcodes of marine organisms from the Hudson-Raritan Estuary in order to monitor its biodiversity. Part of our larger CIVITAS project (see previous post), these scholars have collected samples of algae and invertebrates from our Harlem River expeditions to create a baseline of what’s living there today. This type of work requires tedious pipetting, labeling on tiny vials, centrifuging, vortexing, and other crucial steps just to be able to extract the DNA from the organisms’ cells. During our next lab, these scholars will amplify the DNA and run it through a gel electrophoresis in order to prepare for genetic sequencing. The last step will be to identify the species using bioinformatics. You can see their research proposals here. A big thanks to our sponsor organization The Urban Barcode Project and Dr. Christine Marizzi from Cold Spring Harbor Lab for her and her team’s support.